Development of Microsatellite Loci in Scrophularia incisa (Scrophulariaceae) and Cross-Amplification in Congeneric Species
نویسندگان
چکیده
منابع مشابه
Development of microsatellite loci in Scrophularia incisa (Scrophulariaceae) and cross-amplification in congeneric species1
UNLABELLED PREMISE OF THE STUDY To elucidate the population genetics and phylogeography of Scrophularia incisa, microsatellite primers were developed. We also applied these microsatellite markers to its closely related species S. dentata and S. kiriloviana. • METHODS AND RESULTS Using the compound microsatellite marker technique, 12 microsatellite primers were identified in S. incisa. The ...
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UNLABELLED PREMISE OF THE STUDY Microsatellite loci from a genomic library of the species Narcissus papyraceus were optimized and characterized for studies of population genetics. • METHODS AND RESULTS Eleven markers that were successfully amplified showed polymorphism when tested on 50 individuals from two populations in southern Spain and northern Morocco. Overall, the number of alleles ...
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UNLABELLED PREMISE OF THE STUDY Microsatellite loci were isolated and characterized from enriched genomic libraries of Artocarpus altilis (breadfruit) and tested in four Artocarpus species and one hybrid. The microsatellite markers provide new tools for further studies in Artocarpus. • METHODS AND RESULTS A total of 25 microsatellite loci were evaluated across four Artocarpus species and o...
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Polyploidy plays an important role in the evolution of plant genomes. To enable the investigation of the polyploidy events within the genus Anemone, we developed eighteen microsatellite markers from the hexaploid species A. amurensis (Ranunculaceae), and tested their transferability in five closely related species. The number of total alleles (N(A)) for each resulting locus varied from one to e...
متن کاملPolymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species.
Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 microsatellite loci ...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2014
ISSN: 2168-0450
DOI: 10.3732/apps.1300077